CDC Culturable Methods
M210 Level 1 – Presumptive Legionella Detection – Presumptive enumeration or presence/absence
M211 Level 2– Identification, Enumeration, and individual serotyping of L. pneumophila, Serogroups 1-14
M212 Level 3 – Identification and Enumeration of L. pneumophila plus L. anisa, L. bozmanii, L. dumoffii L. gormanii, L. jordanis, L. longbeachae, L. micdadei, L. macheachernii, L. sainthelensis. Individual serotyping included.
M213 Level 4 – CDC Heat Enrichment Plus Level 3 Identification for Samples Suspected of Containing High Levels of Protozoans
M 214 Pure Culture Preparation and Storage
M 215 Pure Culture Preparation and Shipping
Polymerase Chain Reaction (PCR) Legionella Panels
M163 – Broad Screen Legionella Panel
Presence/absence of 34 species of Legionella including L. pneumophila. Species are combined and include: L. pneumophila : 16S rRNA gene and mip gene, L. anisa, L. oakridgensis, L. bozemanii, L. birminghamensis, L, brunensis,L. cherri, . L. cincinnatiensis, . L. dumoffii, L. erythra, L. fairfieldensis, L. feelei, L. gormanii, L. gratiana, L. hackeliae, L. israelensis, L. jamestownensis. L. jordanis, L. lansingensis, L. longbeachae, L. maceachernii, L. micdadei, L. moravica, L. parisiensis, L. quinlivanii, L. rubrilucens, L. santicrucis, L. sainthelensis, L. spiritensis, L. steigerwaltii, L. tucsonensis. L. wadsworthii, L. worsliensis
Polymerase Chain Reaction (PCR) Legionella Panels
The test codes below include M212 Level 3 culture, if positive*
M162 Presence/absence of L. pneumophila, L. micdadei, L. maceachernii, L. sainthelensis/cincinnatiensis.
M103 Presence/absence of L. pneumophila
M102 Presence/absence of L. micdadei
M104 Presence/absence of L. sainthelensis/cincinnatiensis
M101Presence/absence of L. maceachernii
Legionella pneumophila serotype 1 Strain Identification
M029 DNA Sequence Based Typing
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An Overview of Legionella Analyses
By Diane Miskowski, MPH EMSL Analytical, Inc.
The first recognized outbreak of Legionnnaires' Disease occurred in the US at the American Legion Convention in Philadelphia during the summer of 1976. read more...
For additional information please click the links below.
Legionnaires' Disease
Clinical Microbiology Reviews
Cooling Technology Institute
more resources...
Legionella images courtesy of CDC, Public Health Image Library (PHIL)